Changes in the normal redox state of cells can cause cell toxicity, induced directly by the reactive oxygen, or from its derivatives like superoxide radicals, hydroxyl radicals or peroxides. These types of molecules can generate damage to all cell components–proteins, lipids, and DNA–if not offset by specific antioxidant action.

The human spermatozoon is highly susceptible to oxidative stress. Peroxidative damage directly affects the lipid component of the membrane and also generates breaks in the chains of both nuclear and mitochondrial DNA.

Applications:

• oxiSperm®, for its clinical application, is an easy-to-perform and reliable assay that allows the measurement of potential excess superoxide anions present in the ejaculate, with a minimum volume of sperm.
• Determinación de los niveles de oxidación en el eyaculado y re-análisis tras el tratamiento con antioxidantes.
• Evaluation of the oxidation in each patient to select the best sample that can be presented.

La entrada oxiSperm® se publicó primero en halotech® DNA.

La entrada fluored® se publicó primero en halotech® DNA.

La entrada fluogreen® se publicó primero en halotech® DNA.

Its use is advisable for patients with progressive motility below 40% to evaluate the proportion of live and dead spermatozoa. This test is used as a check on the motility evaluation, as the percentage of dead cells should not exceed that of immotile spermatozoa.

Cases of necrozoospermia are related to epididymal pathology (Wilton et al, 1988; Correa-Pérez et al, 2004). A high percentage of vital but immotile spermatozoa is indicative of structural defects in the flagellum (Chemes and Rawe, 2003).

Applications of vitalTest®

• Immediate determination* of the quality of the membrane of spermatozoa in the ejaculate.
• Determination of necrozoospermia.
• The incubation of sperm with the reagent is not necessary.

La entrada vitalTest® se publicó primero en halotech® DNA.

dyn-halosperm® allows medical professionals to add more information to the clinical diagnosis at the time of selecting the reproductive technique best suited to each couple. For example, for a patient with a high increase in the degree of DNA fragmentation in the first few minutes after ejaculating, it would be advisable to direct him to a technique that ensures short periods of in vitro manipulation and rapid fertilisation of the oocyte.

In this way, dyn-halosperm® is useful for selecting the optimal timing of the assisted reproduction cycle, evaluating the quality of the sperm sample and providing answers to cases of idiopathic infertility or repeated miscarriages, among other causes.

La entrada dyn-halosperm® se publicó primero en halotech® DNA.

Troubleshooting:

Conventional semen analysis does not consider the assessment of a critical parameter that affects male fertility such as pro-oxidant activity at the ejaculate. oxiSperm® II fills this gap, offering the possibility of determining pro-oxidant activity in neat semen samples, seminal plasma and spermatozoa.

Key Benefits:

Fast, easy and reproducible: oxiSperm® II enables the quick and user-friendly measurement of pro-oxidant activity, reducing the need for complex laboratory equipment.
Enhanced Assessment: Complementing conventional sperm analysis, oxiSperm® II provides valuable information about sperm quality and helps medical professionals to determine when patients require treatment with antioxidant supplements.

oxiSperm® II is based on the NBT reaction in the form of a pre-assembled and specifically formulated reactive membrane. The interaction of the sample with the reactive membrane results in the appearance of color in the sample, ranging from pale pink to purple/dark blue, which can be easily and comparatively quantified compared to the color palette provided with the kit.

Product Features:
Comprehensive evaluation of pro-oxidant activity: oxiSperm® II allows the evaluation of pro-oxidant activity in neat semen samples, seminal plasma, or spermatozoa in a simple, fast, and reliable way.

Clinical Applications:
Medical professionals use Halotech’s solutions, including oxiSperm® II, to complement conventional semen analysis.
Evaluation of pro-oxidant activity in ejaculation is particularly recommended in several scenarios, including the following:

Men presenting altered conventional semen analysis.
Men with altered level of sperm DNA fragmentation.
Unexplained infertility.
Men over the age of 40.
Factors such as history of cancer, use of medications, exposure to toxic agents, urogenital infections, and unhealthy lifestyle habits.

Conclusion:
oxiSperm® II allows medical professionals to comprehensively assess sperm quality by assessing pro-oxidant activity in the ejaculate. By bridging the gap in conventional semen analysis, this innovative diagnostic kit can help optimize treatment decisions and improve reproductive outcomes.

La entrada oxiSperm® II se publicó primero en halotech® DNA.

The key advantage of the halosperm® G2 PRO kit is that it only takes 10 minutes to process the sample, with no need to work in an extraction hood.

La entrada halosperm® G2 PRO se publicó primero en halotech® DNA.

halosperm® is based on the SCD technique, patented by halotech®, based on a controlled DNA denaturation process to facilitate the subsequent removal of the proteins contained in each spermatozoon. In this way, normal spermatozoa create halos formed by loops of DNA at the head of the sperm, which are not present in those with damaged DNA.

The images of halos generated with halosperm® are highly contrasted and can be evaluated precisely using conventional or fluorescent microscopy. The tails of the spermatozoa are preserved, so it is possible to discriminate them from other cell types that might be present in the ejaculate, such as leukocytes. In addition, halosperm® allows the visualisation of spermatozoa that contain highly degraded DNA compared to other types of less aggressive damage. This fact is of great importance in the diagnosis and monitoring of varicocele.

Medical professionals use information from halotech® solutions at the start of treatment, supplementing conventional semen analysis. Sperm DNA damage is a multifactorial process. Because it can have multiple causes, fragmentation, oxidation, and vitality analysis is especially recommended for:

• Couples with a history of miscarriages
• Couples with unexplained infertility for more than 6 months
• Men older than 40
• Use of tight-fitting clothing
• Men with a history of cancer
• Men under treatment with prescription medications
• Men exposed to toxic agents
• Men who have had urogenital infections
• Men with unhealthy lifestyles (smoking, poor diet, obesity, sedentary)
• Men with unhealthy lifestyle habits: those who smoke, are sedentary, have an unbalanced diet or are obese
• Male factor of unknown cause
• Poor quality of the embryo during second cycles of egg donation

halosperm® G2 measures DNA fragmentation in a fast, easy way, similar to halosperm®, but also partially eliminates the bad odour generated by the lysis. By using a few drops, the test will offer the same result as the halosperm® test, maintaining its simplicity and ease of use.

halosperm® G2 also includes staining solutions for bright field microscopy.

La entrada halosperm® G2 se publicó primero en halotech® DNA.

halosperm® is based on the SCD technique, patented by halotech®, based on a controlled DNA denaturation process to facilitate the subsequent removal of the proteins contained in each spermatozoon. In this way, normal spermatozoa create halos formed by loops of DNA at the head of the sperm, which are not present in those with damaged DNA.

The images of halos generated with halosperm® are highly contrasted and can be evaluated precisely using conventional or fluorescent microscopy. The tails of the spermatozoa are preserved, so it is possible to discriminate them from other cell types that might be present in the ejaculate, such as leukocytes. In addition, halosperm® allows the visualisation of spermatozoa that contain highly degraded DNA compared to other types of less aggressive damage. This fact is of great importance in the diagnosis and monitoring of varicocele.

Medical professionals use information from halotech® solutions at the start of treatment, supplementing conventional semen analysis. Sperm DNA damage is a multifactorial process. Because it can have multiple causes, fragmentation, oxidation, and vitality analysis is especially recommended for:

• Couples with a history of miscarriages
• Couples with unexplained infertility for more than 6 months
• Men older than 40
• Use of tight-fitting clothing
• Men with a history of cancer
• Men under treatment with prescription medications
• Men exposed to toxic agents
• Men who have had urogenital infections
• Men with unhealthy lifestyles (smoking, poor diet, obesity, sedentary)
• Men with unhealthy lifestyle habits: those who smoke, are sedentary, have an unbalanced diet or are obese
• Male factor of unknown cause
• Poor quality of the embryo during second cycles of egg donation

La entrada halosperm® se publicó primero en halotech® DNA.